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This was not the first time that microbiologists experienced problems with agar. A phenomenon called “The Great Plate Count Anomaly” baffled researchers in the early 20th century when they observed that the number of cells seen under a microscope didn’t match the actual number of colonies growing on an agar plate. Investigating this discrepancy, researchers found agar itself to be the culprit: when nutrient broths are heated with agar during boiling, harmful byproducts (hydroperoxide) can form due to the reaction of agar with phosphate minerals contained in the media. Researchers can avoid this by autoclaving agar separately from the nutrient broth, or by reducing the amount of agar used.，这一点在体育直播中也有详细论述
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We recruited 557 participants from Prolific (277 male, 271 female, 9 self-identify; MageM_{\text{age}} = 42.92 years, SDSD = 13.83, range: 18-82). The sample was 63% White, 13% Black, 11% Latin American, 6% Multi-Racial, 4% East Asian, and 3% other ethnicities. Of these, 504 participants (90.5%) provided a final hypothesis and were included in discovery rate analyses, while 512 participants (91.9%) provided a final likelihood rating and were included in confidence change analyses. Participants who exited the chatbot interface after providing their hypothesis but before rating their final confidence were excluded from confidence analyses only. All participants who completed the study provided informed consent and were paid $1.10. The study took the median participant 5.4 minutes. The study was approved by an Institutional Review Board.
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